政大機構典藏-National Chengchi University Institutional Repository(NCCUR):Item 140.119/61796

English | 正體中文 | 简体中文 | Post-Print筆數 : 27 | Items with full text/Total items : 113485/144472 (79%)
Visitors : 51389904 Online Users : 356

RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.

Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer

政大機構典藏 > 理學院 > 神經科學研究所 > 期刊論文 > Item 140.119/61796

Please use this identifier to cite or link to this item: https://nccur.lib.nccu.edu.tw/handle/140.119/61796

Title:	Protein Kinase CK2 Enhances Mcl-1 Gene Expression through the SRF-Mediated Pathway in the Rat Hippocampus
Authors:	趙知章 Chang,Chia-Ming;Chao,Chih-Chang
Contributors:	神科所
Keywords:	protein kinase CK2;serum response factor;Mcl-1;antiapoptosis
Date:	2013-06
Issue Date:	2013-11-22 15:17:46 (UTC+8)
Abstract:	The protein kinase CK2 (casein kinase 2) is a ubiquitous serine/threonine protein kinase that suppresses apoptosis. CK2 is composed of catalytic and regulatory subunits, and CK2-dependent phosphorylation is a global mechanism in the inhibition of caspase signaling pathways. The serum response factor (SRF) is an important regulator of cell growth and differentiation. Although CK2 has been shown to phosphorylate SRF in vitro, the biological relevance of this interaction remains largely unclear. We observed increased SRF phosphorylation and increased Mcl-1 gene expression in hippocampal CA1 neurons following transfection with a plasmid expressing the wild-type CK2α (CK2αWT) protein, whereas transfection with a plasmid expressing a catalytically inactive mutant of CK2α (CK2α156A) reduced Mcl-1 gene expression. Cotransfection with a plasmid expressing the inactive SRF99A mutant inhibited the CK2αWT-induced upregulation of Mcl-1 gene expression. The expression of either the CK2α156A or the SRF99A mutant also inhibited the glutamate-induced upregulation of Mcl-1 protein expression in PC12 cells. Our results suggest that CK2-mediated signaling represents a cellular mechanism that may aid in the development of alternative therapeutic strategies to attenuate apoptosis in hippocampal neurons.
Relation:	Journal of Neuroscience Research, 91(6), 808-817
Data Type:	article
DOI 連結:	http://dx.doi.org/10.1002/jnr.23212
DOI:	10.1002/jnr.23212
Appears in Collections:	[神經科學研究所] 期刊論文

Files in This Item:

File	Description	Size	Format
808-817.pdf		527Kb	Adobe PDF2	1084	View/Open

社群 sharing

著作權政策宣告 Copyright Announcement

1.本網站之數位內容為國立政治大學所收錄之機構典藏，無償提供學術研究與公眾教育等公益性使用，惟仍請適度，合理使用本網站之內容，以尊重著作權人之權益。商業上之利用，則請先取得著作權人之授權。
The digital content of this website is part of National Chengchi University Institutional Repository. It provides free access to academic research and public education for non-commercial use. Please utilize it in a proper and reasonable manner and respect the rights of copyright owners. For commercial use, please obtain authorization from the copyright owner in advance.

2.本網站之製作，已盡力防止侵害著作權人之權益，如仍發現本網站之數位內容有侵害著作權人權益情事者，請權利人通知本網站維護人員(nccur@nccu.edu.tw)，維護人員將立即採取移除該數位著作等補救措施。
NCCU Institutional Repository is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff(nccur@nccu.edu.tw). We will remove the work from the repository and investigate your claim.

Loading...