政大機構典藏-National Chengchi University Institutional Repository(NCCUR):Item 140.119/64582

English | 正體中文 | 简体中文 | Post-Print筆數 : 27 | Items with full text/Total items : 112721/143689 (78%)
Visitors : 49616450 Online Users : 875

RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.

Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer

政大機構典藏 > 商學院 > 風險管理與保險學系 > 期刊論文 > Item 140.119/64582

Please use this identifier to cite or link to this item: https://nccur.lib.nccu.edu.tw/handle/140.119/64582

Title:	Unique marker finder algorithm generates molecular diagnostic markers
Authors:	Chiu, Sung-Kay;Hsieh, Ming-Hua;Tzeng, Chi-Meng 謝明華;曾驥孟
Contributors:	風管系
Keywords:	comparative genomics;subtraction;diagnostic markers;micro-array;real-time PCR.
Date:	2011-03
Issue Date:	2014-03-11 17:38:43 (UTC+8)
Abstract:	By taking advantage of the power of comparative genomics, we devised an algorithm, Unique Marker Finder (U-MarFin), to generate a collection of unique DNA sequences from a target organism. The whole target genome is partitioned into a scoring pool of less 4000 base-pair fragments, which are then subjected to elimination of homologous sequences in other bacterial genomes by BLAST alignment, and looked for all open reading frames as they may be applied as unique markers. Through regular, nested, multiplex and real time PCR and microarray technology, we empirically demonstrated that the sequences discovered were highly specific to the species that they are derived from, and they can serve as molecular biomarkers for diagnostic purpose.
Relation:	International Journal of Bioinformatics Research and Applications, 7(1), 24-42
Data Type:	article
DOI 連結:	http://dx.doi.org/10.1504/IJBRA.2011.039168
DOI:	10.1504/IJBRA.2011.039168
Appears in Collections:	[風險管理與保險學系] 期刊論文

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML2	1381	View/Open

社群 sharing

著作權政策宣告 Copyright Announcement

1.本網站之數位內容為國立政治大學所收錄之機構典藏，無償提供學術研究與公眾教育等公益性使用，惟仍請適度，合理使用本網站之內容，以尊重著作權人之權益。商業上之利用，則請先取得著作權人之授權。
The digital content of this website is part of National Chengchi University Institutional Repository. It provides free access to academic research and public education for non-commercial use. Please utilize it in a proper and reasonable manner and respect the rights of copyright owners. For commercial use, please obtain authorization from the copyright owner in advance.

2.本網站之製作，已盡力防止侵害著作權人之權益，如仍發現本網站之數位內容有侵害著作權人權益情事者，請權利人通知本網站維護人員(nccur@nccu.edu.tw)，維護人員將立即採取移除該數位著作等補救措施。
NCCU Institutional Repository is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff(nccur@nccu.edu.tw). We will remove the work from the repository and investigate your claim.

Loading...