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    Please use this identifier to cite or link to this item: http://nccur.lib.nccu.edu.tw/handle/140.119/65233


    Title: A gene delivery system for insect cells mediated by arginine-rich cell-penetrating peptides
    Authors: Chen, Yung-Jen;Liu, Betty Revon;Dai, Yun-Hao;Lee, Cheng-Yi;Chan, Ming-Huan;Chen, Hwei-Hsien;Chiang, Huey-Jenn;Lee, Han-Jung
    詹銘煥
    Contributors: 神科所
    Keywords: Cell-penetrating peptide (CPP);Cellular internalization;Polyarginine;Protein transduction;Spodoptera frugiperda;Transgenic insects
    Date: 2012.02
    Issue Date: 2014-04-09 17:18:18 (UTC+8)
    Abstract: Most bioactive macromolecules, such as protein, DNA and RNA, basically cannot permeate into cells freely from outside the plasma membrane. Cell-penetrating peptides (CPPs) are a group of short peptides that possess the ability to traverse the cell membrane and have been considered as candidates for mediating gene and drug delivery into living cells. In this study, we demonstrate that three arginine-rich CPPs (SR9, HR9 and PR9) are able to form stable complexes with plasmid DNA and deliver DNA into insect Sf9 cells in a noncovalent manner. The transferred plasmid DNA containing enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) coding regions could be expressed in cells functionally assayed at both the protein and RNA levels. Furthermore, treatment of cells with CPPs and CPP/DNA complexes resulted in a viability of 84–93% indicating these CPPs are not cytotoxic. These results suggest that arginine-rich CPPs appear to be a promising tool for insect transgenesis
    Relation: Gene,493(2), 201-210
    Source URI: http://dx.doi.org/10.1016/j.gene.2011.11.060
    Data Type: article
    Appears in Collections:[神經科學研究所 ] 期刊論文

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