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    Please use this identifier to cite or link to this item: http://nccur.lib.nccu.edu.tw/handle/140.119/65236


    Title: mGluR5 positive modulators both potentiate activation and restore inhibition in NMDA receptors by PKC dependent pathway
    Authors: Chen, Hwei-Hsien;Liao, Pei-Fei;Chan, Ming-Huan
    詹銘煥
    Contributors: 神科所
    Date: 2011.02
    Issue Date: 2014-04-09 17:44:09 (UTC+8)
    Abstract: Background
    In order to understand the interaction between the metabotropic glutamate subtype 5 (mGluR5) and N-methyl-D-aspartate (NMDA) receptors, the influence of mGluR5 positive modulators in the inhibition of NMDA receptors by the noncompetitive antagonist ketamine, the competitive antagonist D-APV and the selective NR2B inhibitor ifenprodil was investigated.

    Methods
    This study used the multi-electrode dish (MED) system to observe field potentials in hippocampal slices of mice.

    Results
    Data showed that the mGluR5 agonist (RS)-2-chloro-5-hydroxyphenylglycine (CHPG), as well as the positive allosteric modulators 3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl) benzamide (CDPPB) and 3,3'-difluorobenzaldazine (DFB) alone did not alter the basal field potentials, but enhanced the amplitude of field potentials induced by NMDA. The inhibitory action of ketamine on NMDA-induced response was reversed by CHPG, DFB, and CDPPB, whereas the blockade of NMDA receptor by D-APV was restored by CHPG and CDPPB, but not by DFB. Alternatively, activation of NMDA receptors prior to the application of mGluR5 modulators, CHPG was able to enhance NMDA-induced field potentials and reverse the suppressive effect of ketamine and D-APV, but not ifenprodil. In addition, chelerythrine chloride (CTC), a protein kinase C (PKC) inhibitor, blocked the regulation of mGluR5 positive modulators in enhancing NMDA receptor activation and recovering NMDA receptor inhibition. The PKC activator (PMA) mimicked the effects of mGluR5 positive modulators on enhancing NMDA receptor activation and reversing NMDA antagonist-evoked NMDA receptor suppression.

    Conclusion
    Our results demonstrate that the PKC-dependent pathway may be involved in the positive modulation of mGluR5 resulting in potentiating NMDA receptor activation and reversing NMDA receptor suppression induced by NMDA antagonists.
    Relation: Journal of Biomedical Science,18(1), 19
    Data Type: article
    DOI 連結: http://dx.doi.org/10.1186/1423-0127-18-19
    DOI: 10.1186/1423-0127-18-19
    Appears in Collections:[神經科學研究所 ] 期刊論文

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