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    题名: Effects of cis and trans regulatory variations on the expression divergence of heat shock response genes between yeast strains
    作者: 李靜敏
    Li, Ching-Min
    曾正男
    Tzeng, Jeng-Nan
    宋皇模
    Sung, Huang-Mo
    贡献者: 應數系
    关键词: Yeast;Transcription regulation;Stress;Heat-shock;cis regulatory variation;trans regulatory variation
    日期: 2012.09
    上传时间: 2014-05-22 11:14:38 (UTC+8)
    摘要: Phenotypic variation among individuals in a population can be due to DNA sequence variation in protein coding regions or in regulatory elements. Recently, many studies have indicated that mutations in regulatory elements may be the major cause of phenotypic evolution. However, the mechanisms for evolutionary changes in gene expression are still not well understood. Here, we studied the relative roles of cis and trans regulatory changes in Saccharomyces cerevisiae cells to cope with heat stress. It has been found that the expression level of ~ 300 genes was induced at least two fold and that of ~ 500 genes was repressed at least two fold in response to heat shock. From the former set of genes, we randomly selected 65 genes that showed polymorphism(s) between the BY and RM strains for pyrosequencing analysis to explore the relative contributions of cis and trans regulatory variations to the expression divergence between BY and RM. Our data indicated that the expression divergence between BY and RM was mainly due to trans regulatory variations under either the normal condition or the heat stress condition. However, the relative contribution of trans regulatory variation was decreased from 76.9% to 61.5% after the heat shock stress. These results indicated that the cis regulatory variation may play an important role in the adaption to heat stress. In our data, 43.1% (28 genes) of the 65 genes showed the same trend of cis or trans variation effect after the heat shock stress, 35.4% (23 genes) showed an increased cis variation effect and 21.5% (14 genes) showed an increased trans variation effect after the heat shock stress. Thus, our data give insights into the relative roles of cis and trans variations in response to heat shock in yeast.
    關聯: Gene, 506(1), 93-97
    数据类型: article
    ISBN: 10.1016/j.gene.2012.06.034
    DOI 連結: http://dx.doi.org/10.1016/j.gene.2012.06.034
    显示于类别:[應用數學系] 期刊論文

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