English  |  正體中文  |  简体中文  |  Post-Print筆數 : 11 |  Items with full text/Total items : 89686/119522 (75%)
Visitors : 23949723      Online Users : 340
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://nccur.lib.nccu.edu.tw/handle/140.119/75385


    Title: Andrographolide enhances nuclear factor-κB subunit p65 Ser 536 dephosphorylation through activation of protein phosphatase 2A in vascular smooth muscle cells
    Authors: Hsieh, C.Y.;Hsu, M.J.;Hsiao, G.;Wang, Yi-hsuan;Huang, C.W.;Chen, S.W.;Jayakumar, T.;Chiu, P.T.;Chiu, Y.H.;Sheu, J.R.
    Contributors: 法律科際整合研究所
    Keywords: Andrographolide;Anti-inflammatory action;Ceramides;Dephosphorylations;DNA binding activity;Down-regulation;Injury models;Matrix metalloprotease;Neointimal formation;Nitric-oxide synthase;Nuclear factors;Nuclear translocations;Pro-inflammatory stimuli;Protein phosphatase 2A;Selective inhibition;Sphingomyelinase;Vascular smooth muscle cells;Amides;Cells;Hydrolases;Lead oxide;Muscle;Phosphatases;Phosphorylation;Signaling;Transcription factors;Blood vessel prostheses;andrographolide;ceramide;DNA;gamma interferon;gelatinase B;I kappa B alpha;I kappa B kinase;inducible nitric oxide synthase;lipopolysaccharide;phosphoprotein phosphatase 2A;serine;sphingomyelin phosphodiesterase;transcription factor RelA;animal cell;animal experiment;animal model;animal tissue;article;carotid artery injury;controlled study;down regulation;drug effect;drug mechanism;enzyme activation;enzyme activity;male;neointima;nonhuman;priority journal;protein degradation;protein dephosphorylation;protein DNA binding;protein expression;protein phosphorylation;protein transport;rat;reporter gene;signal transduction;smooth muscle fiber;vascular smooth muscle;Active Transport, Cell Nucleus;Andrographis;Animals;Anti-Inflammatory Agents, Non-Steroidal;Cell Nucleus;Ceramides;Diterpenes;Enzyme Activation;Interferon-gamma;Lipopolysaccharides;Male;Matrix Metalloproteinase 9;Muscle, Smooth, Vascular;Myocytes, Smooth Muscle;Nitric Oxide Synthase Type II;Phosphorylation;Protein Phosphatase 2;Rats;Rats, Wistar;Serine;Sphingomyelin Phosphodiesterase;Transcription Factor RelA;Andrographis;Rattus
    Date: 2011-02
    Issue Date: 2015-05-29 16:54:58 (UTC+8)
    Abstract: Recent studies have demonstrated that transcription factor nuclear factor (NF)-κB inhibition may contribute to the protective anti-inflammatory actions of andrographolide, an abundant component of plants of the genus Andrographis. However, the precise mechanism by which andrographolide inhibits NF-κB signaling remains unclear. We thus investigated the mechanism involved in andrographolide suppression of NF-κB signaling in rat vascular smooth muscle cells (VSMCs) exposed to proinflammatory stimuli, LPS, and IFN-γ. Andrographolide was shown to suppress LPS/IFN-γ-induced inducible nitric-oxide synthase and matrix metalloprotease 9 expression in rat VSMCs. Andrographolide also inhibited LPS/IFN-γ-induced p65 nuclear translocation, DNA binding activity, p65 Ser536 phosphorylation, and NF-κB reporter activity. However, IKK phosphorylation and downstream inhibitory κBα phosphorylation and degradation were not altered by the presence of andrographolide in LPS/IFN-γ-stimulated VSMCs. These andrographolide inhibitory actions could be prevented by selective inhibition of neutral sphingomyelinase and protein phosphatase 2A (PP2A). Furthermore, andrographolide was demonstrated to increase ceramide formation and PP2A activity in VSMCs and to inhibit neointimal formation in rat carotid injury models. These results suggest that andrographolide caused neutral sphingomyelinase-mediated ceramide formation and PP2A activation to dephosphorylate p65 Ser536, leading to NF-κB inactivation and subsequent inducible nitric-oxide synthase down-regulation in rat VSMCs stimulated by LPS and IFN-γ. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
    Relation: Journal of Biological Chemistry, 286(8), 5942-5955
    Data Type: article
    DOI 連結: http://dx.doi.org/10.1074/jbc.M110.123968
    DOI: 10.1074/jbc.M110.123968
    Appears in Collections:[法律與科技整合研究所] 期刊論文

    Files in This Item:

    File Description SizeFormat
    Hsieh-5942-55.pdf4711KbAdobe PDF1144View/Open


    All items in 政大典藏 are protected by copyright, with all rights reserved.


    社群 sharing

    著作權政策宣告
    1.本網站之數位內容為國立政治大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,惟仍請適度,合理使用本網站之內容,以尊重著作權人之權益。商業上之利用,則請先取得著作權人之授權。
    2.本網站之製作,已盡力防止侵害著作權人之權益,如仍發現本網站之數位內容有侵害著作權人權益情事者,請權利人通知本網站維護人員(nccur@nccu.edu.tw),維護人員將立即採取移除該數位著作等補救措施。
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback