English  |  正體中文  |  简体中文  |  Items with full text/Total items : 88295/117812 (75%)
Visitors : 23397619      Online Users : 60
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://nccur.lib.nccu.edu.tw/handle/140.119/75650


    Title: Inhibition of vascular smooth muscle cell proliferation by the vitamin E derivative pentamethylhydroxychromane in an in vitro and in vivo study: pivotal role of hydroxyl radical-mediated PLCγ1 and JAK2 phosphorylation
    Authors: Hsieh, C.-Y.;Liu, C.-L.;Wang, Yi Hsuan
    Contributors: 法科所
    Keywords: 6 hydroxy 2,2,5,7,8 pentamethylchroman;alpha tocopherol;deferoxamine;ferrous ion;hydroxyl radical;Janus kinase 2;mitogen activated protein kinase 1;mitogen activated protein kinase 3;phospholipase C gamma1;protein kinase B;protein kinase C alpha;protein kinase C delta;reactive oxygen metabolite;recombinant platelet derived growth factor BB;STAT3 protein;animal cell;animal experiment;artery intima proliferation;article;carotid artery injury;cell cycle arrest;cell cycle G0 phase;cell cycle G1 phase;cell cycle G2 phase;cell cycle M phase;cell cycle progression;cell stimulation;common carotid artery;controlled study;dose response;drug effect;drug mechanism;electron spin resonance;enzyme inhibition;enzyme phosphorylation;Fenton reaction;in vitro study;in vivo study;male;nonhuman;percutaneous transluminal angioplasty;priority journal;rat;smooth muscle fiber;vascular smooth muscle;Animals;Antioxidants;Benzopyrans;Cell Cycle;Cell Proliferation;Cells, Cultured;Down-Regulation;Drug Evaluation, Preclinical;Hydroxyl Radical;Janus Kinase 2;Male;Muscle, Smooth, Vascular;Myocytes, Smooth Muscle;Phospholipase C gamma;Phosphorylation;Rats;Rats, Wistar;Reactive Oxygen Species;Vitamin E;Rattus
    Date: 2010-09
    Issue Date: 2015-06-10 15:05:52 (UTC+8)
    Abstract: Abnormal proliferation of vascular smooth muscle cells (VSMCs) plays an important role in the development of cardiovascular diseases. PMC (2,2,5,7,8-pentamethyl-6-hydroxychromane) is the most potent hydrophilic derivative of vitamin E. In this study, we investigated the mechanisms of PMC inhibition of VSMC proliferation in vitro and in vivo. PMC (20 and 50γM) obviously suppressed proliferation of PDGF-BB-stimulated cells, but not resting cells, and arrested cell cycle progression at the G2/M phase. A significant reduction in neointimal formation in carotid arteries was observed in PMC (5mg/kg/day)-treated rats after balloon angioplasty. Activation of STAT3, JAK2, PLCγ1, PKCγ, and ROS, but not ERK1/2, AKT, or PKCγ, was markedly inhibited by PMC in PDGF-BB-stimulated VSMCs. Deferoxamine and PMC significantly inhibited the phosphorylation of PLCγ1 and JAK2 and arrested cell cycle progression at the G2/M phase. These events, however, were reversed in the presence of Fe2+. Moreover, PMC directly inhibited hydroxyl radical formation in both the Fenton reaction and VSMCs according to an electron spin resonance study. In conclusion, this study demonstrates for the first time that PMC inhibits VSMC proliferation in vitro and balloon injury-induced neointimal formation in vivo. The inhibitory mechanism of PMC may involved the inhibition of hydroxyl radical-mediated PLCγ1-PKCγ and JAK2-STAT3 activation and causes cell cycle arrest at the G2/M phase. PMC treatment may represent a novel approach for lowering the risk of or improving function in abnormal VSMC proliferation-related vascular diseases. © 2010 Elsevier Inc.
    Relation: Free Radical Biology and Medicine, Volume 49, Issue 5, Pages 881-893
    Data Type: article
    DOI 連結: http://dx.doi.org/http://dx.doi.org/10.1016/j.freeradbiomed.2010.06.014
    DOI: 10.1016/j.freeradbiomed.2010.06.014
    Appears in Collections:[法律與科技整合研究所] 期刊論文

    Files in This Item:

    File Description SizeFormat
    881.pdf2479KbAdobe PDF1002View/Open


    All items in 政大典藏 are protected by copyright, with all rights reserved.


    社群 sharing

    著作權政策宣告
    1.本網站之數位內容為國立政治大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,惟仍請適度,合理使用本網站之內容,以尊重著作權人之權益。商業上之利用,則請先取得著作權人之授權。
    2.本網站之製作,已盡力防止侵害著作權人之權益,如仍發現本網站之數位內容有侵害著作權人權益情事者,請權利人通知本網站維護人員(nccur@nccu.edu.tw),維護人員將立即採取移除該數位著作等補救措施。
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback