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政大機構典藏 > 理學院 > 神經科學研究所 > 期刊論文 > Item 140.119/65233

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Title:	A gene delivery system for insect cells mediated by arginine-rich cell-penetrating peptides
Authors:	Chen, Yung-Jen;Liu, Betty Revon;Dai, Yun-Hao;Lee, Cheng-Yi;Chan, Ming-Huan;Chen, Hwei-Hsien;Chiang, Huey-Jenn;Lee, Han-Jung 詹銘煥
Contributors:	神科所
Keywords:	Cell-penetrating peptide (CPP);Cellular internalization;Polyarginine;Protein transduction;Spodoptera frugiperda;Transgenic insects
Date:	2012.02
Issue Date:	2014-04-09 17:18:18 (UTC+8)
Abstract:	Most bioactive macromolecules, such as protein, DNA and RNA, basically cannot permeate into cells freely from outside the plasma membrane. Cell-penetrating peptides (CPPs) are a group of short peptides that possess the ability to traverse the cell membrane and have been considered as candidates for mediating gene and drug delivery into living cells. In this study, we demonstrate that three arginine-rich CPPs (SR9, HR9 and PR9) are able to form stable complexes with plasmid DNA and deliver DNA into insect Sf9 cells in a noncovalent manner. The transferred plasmid DNA containing enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) coding regions could be expressed in cells functionally assayed at both the protein and RNA levels. Furthermore, treatment of cells with CPPs and CPP/DNA complexes resulted in a viability of 84–93% indicating these CPPs are not cytotoxic. These results suggest that arginine-rich CPPs appear to be a promising tool for insect transgenesis
Relation:	Gene,493(2), 201-210
Source URI:	http://dx.doi.org/10.1016/j.gene.2011.11.060
Data Type:	article
Appears in Collections:	[神經科學研究所 ] 期刊論文

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