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| Title: | 利用NMR模擬尋找MEGA-PRESS 在GSH量化的最佳回訊時間
Determination of Optimal TE for GSH Quantification in MEGA-PRESS sequence using NMR Simulation |
| Authors: | 余竣傑
Yu, Jun-Jie |
| Contributors: | 蔡尚岳
Tsai, Shang-Yueh
余竣傑
Yu, Jun-Jie |
| Keywords: | 麩胱甘肽
MEGA-PRESS
回訊時間
磁共振頻譜
FID-A模擬
Glutathione
MEGA-PRESS
Echo time
Magnetic resonance spectroscopy
FID-A simulation |
| Date: | 2025 |
| Issue Date: | 2025-07-01 15:50:14 (UTC+8) |
| Abstract: | 本研究探討使用MEGA-PRESS (MEscher-GArwood Point-Resolved Spectroscopy) 技術進行麩胱甘肽 (Glutathione, GSH) 定量時的最佳回訊時間 (Echo Time, TE) 參數。GSH作為大腦內主要的抗氧化劑之一，其濃度變化與多種神經退行性疾病及精神疾病密切相關，因此準確定量GSH對於相關研究具有重要意義。
然而，在常規¹H MRS頻譜中，GSH的信號與其他代謝物訊號重疊，特別是受到肌酸 (Cr) 和磷酸肌酸 (PCr) 的干擾。雖然MEGA-PRESS技術能夠透過J差異編輯減少這類干擾，但仍面臨N-乙醯天門冬氨酸 (NAA) 和N-乙醯天門冬氨酸麩氨酸 (NAAG) 因共編輯 (Co-editing) 效應產生的干擾問題。
本研究使用FID-A (FID Appliance) 模擬工具箱，基於量子力學密度矩陣方法，模擬了不同NAA與NAAG (total NAA, tNAA)濃度以及不同組織環境條件 (不同線寬展寬值) 下的GSH+NAA+NAAG頻譜變化。研究評估GSH信號強度、負外側峰 (Negative Outer Lobes) 變化以及tNAA干擾程度三個關鍵指標，以確定最佳的TE參數。
模擬結果顯示：(1) 考慮T₂衰減影響後，GSH積分面積在TE=130 ms達到最大值；(2) GSH的負外側峰在TE=120 ms之後趨近消失，TE=130 ms時已近乎消失；(3) 在所有模擬條件下，TE=130 ms的tNAA干擾面積均小於TE=120 ms。此外，較長的TE值還允許使用更長的編輯脈衝時間 (如40 ms)，可進一步降低36.1%至50.3%的tNAA干擾。
綜合以上發現，本研究建議在使用MEGA-PRESS頻譜編輯技術定量GSH時，採用TE=130 ms作為最佳參數，可兼顧GSH信號強度、譜線品質及減少NAA與NAAG的共編輯干擾，提高GSH定量的準確性。
This study investigates the optimal echo time (TE) parameters for quantifying glutathione (GSH) using MEGA-PRESS (MEscher-GArwood Point-Resolved Spectroscopy) technique. As one of the primary antioxidants in the brain, GSH concentration changes are closely associated with various neurodegenerative and psychiatric disorders, making accurate GSH quantification crucial for related research.
In conventional ¹H MRS spectra, GSH signals overlap with other metabolites, particularly creatine (Cr) and phosphocreatine (PCr). While MEGA-PRESS technique can reduce these interferences through J-difference editing, challenges remain due to co-editing effects from N-acetylaspartate (NAA) and N-acetylaspartylglutamate (NAAG).
This research utilized the FID-A (FID Appliance) simulation toolkit, based on quantum mechanical density matrix methods, to simulate GSH+NAA+NAAG spectra under various tNAA(NAA+NAAG )concentrations and tissue environment conditions (different line broadening values). The study evaluated three key indicators: GSH signal intensity, negative outer lobes variation, and tNAA interference level to determine the optimal TE parameter.
Simulation results demonstrated that: (1) Considering T₂ decay effects, GSH integral area reached its maximum at TE=130 ms; (2) GSH negative outer lobes approached disappearance after TE=120 ms and were almost invisible at TE=130 ms; (3) Under all simulated conditions, tNAA interference area at TE=130 ms was consistently smaller than at TE=120 ms. Additionally, longer TE values allowed for extended editing pulse durations (e.g., 40 ms), further reducing tNAA interference by 36.1% to 50.3%.Based on these findings, this study recommends TE=130 ms as the optimal parameter when quantifying GSH using MEGA-PRESS technique, balancing GSH signal strength, spectral quality, and reduced co-editing interference from NAA and NAAG, thereby improving GSH quantification accuracy. |
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| Description: | 碩士
國立政治大學
應用物理研究所
112755010 |
| Source URI: | http://thesis.lib.nccu.edu.tw/record/#G0112755010 |
| Data Type: | thesis |
| Appears in Collections: | [應用物理研究所 ] 學位論文
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